SuRE™ Technology Screening services
Identification of regulatory DNA elements and non-coding genetic variants that underlie traits and diseases in humans, plants and animals.
Cell Therapy
SuRE™ screening to identify promoters for better control over the expression of your transgene.
Non-Coding Genomics
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Assay the functional impact of non-coding mutations for entire genomes or specific mutation panels.
Drug Discovery
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SuRE™ reporter libraries for monitoring of multiple pathways during compound screens.
Recombinant Protein Production
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Dedicated SuRE™ screens in specific production platforms to identify optimal promoters for increased yields.
Our novel technology in 2 steps
The SuRE method is in essence a strategy to analyze millions of DNA elements for their activity as promoters or enhancers in a single experiment. By analyzing ~300 million DNA elements of ~300bp length, an entire human genome can be analyzed exhaustively at an approximate 30 fold coverage.
The only required input is DNA and once a SuRE library is established it can be analyzed and compared to other DNA sources in the controlled environment of any transfectable cell type. Thus, without the requirement of invasive biopsies, any genome of interest can be analyzed in relevant cell types and under relevant (e.g. stimulatory) conditions.
1
Library Construction
We first construct a plasmid library containing millions of DNA elements. A human genome is randomly sheared, size-selected and cloned into a plasmid library that contains no promoter of its own. These plasmids contain a unique 20bp sequence referred to as a barcode which allows us to uniquely identify and follow each cloned DNA element. This library is characterized (i.e. barcodes are associated to their respective DNA element) by paired-end sequencing.
barcoded plasmid
Fragmented human genome
0.2 - 2 kb
barcoded plasmid
Plasmid library containing > 300 million DNA elements
2
Library Expression Profiling
In step 2, the plasmid library is transfected into a cell type of interest. DNA elements containing promoters or enhancers will transcribe the barcode which can now be detected in the RNA by high-throughput sequencing. By comparing these barcode frequencies with the frequencies in the plasmid library, a quantitative map of promoter and enhancer activity can be constructed for the human genome.
Transfection plasmid library into ~1*10 cells
8
Quantify expressed barcodes
Quantify all barcodes
('input')
Ratio
Promoter and enhancer activity for entire genome
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Introducing our experts
We have a team of ten highly skilled and enthusiastic scientists and technicians dedicated to our clients projects. In addition, they work on new developments around our SuRE™ platform to ensure its continuous improvement for pharmaceutical and agricultural applications. The team is led by founder Joris van Arensbergen and biotech entrepreneur Victor Schut.
Recent news
NEWS
PRESS RELEASE - April 2022Annogen signs another collaboration agreement with a Tier 1 agricultural firm to research non-coding regulatory DNA using SuRE™ screening technology.
Annogen, the Amsterdam based biotech company behind the SuRE™ technology for the functional annotation of the non-coding part of the genome, started a collaboration with a Tier 1 agri firm to test non-coding DNA fragments that are predicted to influence the expression of important crop traits. The results are expected to provide possibilities to intentionally upregulate or downregulate the expression of important genes in crops.
NEWS
Novel approach could aid selective breeding in cattle
Researchers are developing a method of identifying specific letters in the DNA code that govern the development of key physical traits, such as productivity or disease tolerance, in livestock.
The study, involving Roslin researchers and their commercial partner Annogen, will focus on identifying sites in cattle DNA linked to important genes for livestock production and welfare.
NEWS
NEWS – Annogen Featured in C2W
SuRE technology finds hidden gene regulation. Many diseases are determined by differences in as yet undiscovered gene regulation. Thanks to plasmids and 'barcodes', the SuRE assay can map this relatively easily and extensively for the entire human genome.
Read this article by Marysa van den Berg on the C2W website.
Vacancy
VACANCY – Part-Time Position Office Manager
We are looking for an energetic and motivated office manager for our growing company. Tasks will be focused on professionalization of our organization including HR, administration, logistics etc. We are looking for someone that can identify tasks him/herself and that enjoys taking the initiative. The job will be for 12-20 hours per week, ideally 3 or 5 half days but this is flexible and can be discussed.
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