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Promoter Optimization

In addition to bespoke promoter screens SuRE™ can also be used to analyse existing promoters sequences or designs by exhaustive saturated mutagenesis to create a new sequence.

Exhaustive saturated mutagenesis creating a new sequence of existing promoters or designs 

There are various reasons to consider changing existing promoters sequences or designs.
 

SuRE™ mutagenesis screen applications

  • Optimization existing promoters

    • E.g. CpG reduction without affecting activity

  • Minimization existing promoters

    • E.g. Truncation screen to identify smaller version of promoter

  • Identify promising bases for gene editing

    • E.g. Test possible edits to reactivate fetal hemoglobin

       

Typically a SuRE™ mutagenesis screen takes 3 months to generate a high resolution result at protein level: 200 measurements per single base change into any other base or a deletion. Screening per region is done as well depending on areas of interest and end goal of the customer.

 

Example of saturating mutagenesis screen on promoter

Saturating screen identifies critical bases and regions, for both activation (above X-axis) and repression (below X-axis).

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