Exhaustive saturated mutagenesis: creating optimized sequences based on existing promoters or designs
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There are various reasons to consider changing existing promoters sequences or designs.
SuRE™ mutagenesis screen applications include:
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Optimization existing promoters
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E.g. CpG reduction without affecting activity
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Minimization existing promoters
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E.g. Truncation screen to identify smaller version of promoter
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Identify promising bases for gene editing
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E.g. Test possible edits to reactivate fetal hemoglobin
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Typically a SuRE™ mutagenesis screen takes 3 months to generate a high resolution result at protein level: 200 measurements per single base change into any other base or a deletion. Screening per region is done as well depending on areas of interest and end goal of the customer.
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An example of a saturation mutagenesis screen on a promoter. Saturating screen identifies critical bases and regions, for both activation (above X-axis) and repression (below X-axis).