AIM™ Integration Site Mapping
Identifying gene insertions and asses their expression levels for > 100,000 integrations in parallel. Using an adaptation of our barcoding strategy and previously established protocols, we scaled a gene-insertion and expression mapping strategy to unprecedented numbers.
This is specifically valuable for cell engineering in (allogeneic) cell therapy development and for improving recombinant protein manufacturing cell lines.
Identifying favourable integration sites and their expression levels for cell engineering
For cell therapy and cell line generation targeted integration or insight in integration site environment and bias can provide more control over therapy efficacy and safety or manufacturing efficiency and yields
Example of applications are:
• Production lines for stable expression
E.g. CHO sites for maximum expression levels
• Production lines for induced expression
E.g. HEK293T sites for induced expression of Rep protein
• Genetic engineering T cells, NK cells, B cells
E.g. Expression upon T-cell activation or in specific lineage
• Reporter lines for drug screens
E.g. Fluorescence upon inflammation, proliferation, apoptosis
HEK293-INDX™ cell line available for co-development
Using AIM™ we have also established HEK293 clones with near-zero baseline expression and high inducibility with added stimulus: HEK293-INDX1TM.
We believe that having production clones with the ability to express a recombinant protein conditionally, allow for robust expansion of stable clones and subsequent high production of protein at scale.
We are open to collaborations and are excited to hear the potential you see in HEK293-INDX1TM or, more generally, in similar approaches applied to other production systems such as SF9, CHO, etc.